Introduction: Autism spectrum disorder entails continuous failure in communication, social interaction, limited interests, and other behavioral symptoms. It is associated with multiple complications and problems and has a poor prognosis. Anthropometry is the science of measuring linear and angular dimensions of body limbs. Therefore, fingers anthropometry or lips print cheiloscopy can be used to identify individuals or to develop medical anthropology research.
 
Materials and Methods: In this study, 37 male children aged 3 to 18 years old with autism spectrum disorders whose diagnoses were approved by a psychiatrist and 43 healthy children from two Fars ethnic groups living in Mashhad were recruited. Anthropometric measures were measured for studying lips print. Pictures were transferred to a PC and Picture Manager Software was used to investigate each lip patterns. Then, each photo was divided into six districts (each lip into three zones). These areas include: upper right (UR), upper middle (UM), upper left (UL), lower right (LR), lower middle (LM), and lower left (LL). Data were analyzed through polynomial regression and ANOVA models by SPSS software.
 
Results: The most abundant lip patterns in the male population with Autism Spectrum Disorder in Fars ethnicity were complete branch pattern and reticular pattern
 
Conclusion: It seems that lips print cheiloscopy can be used to identify individuals with autism spectrum disorders through more researches.